Volume 1.24 | Sep 4

Intestinal Cell News 1.24 September 4, 2015
Intestinal Cell News
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Noncanonical Role of Transferrin Receptor 1 Is Essential for Intestinal Homeostasis
Scientists inactivated the transferrin receptor 1 gene selectively in murine intestinal epithelial cells. They observed impaired proliferation of intestinal epithelial cell progenitors, aberrant lipid handling, increased mRNA expression of stem cell markers, and striking induction of many genes associated with epithelial-to-mesenchymal transition. [Proc Natl Acad Sci USA] Abstract | Full Article
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PUBLICATIONS (Ranked by impact factor of the journal)

RNA Binding Protein RBM3 Increases β-Catenin Signaling to Increase Stem Cell Characteristics in Colorectal Cancer Cells
Researchers investigated the role of the RNA binding protein RBM3 in stem cell homeostasis in colorectal cancers. Using a doxycycline inducible RBM3 overexpressing cell lines HCT 116 and DLD-1, they measured changes in side population cells that have high xenobiotic efflux capacity and increased capacity for self-renewal. [Mol Carcinog] Abstract

“Store-Operated” cAMP Signaling Contributes to Ca2+-Activated Cl Secretion in T84 Colonic Cells
Investigators assessed the degree to which store-operated cAMP signaling participates in Ca2+-activated Cl transport as measured by transepithelial short-circuit current in polarized T84 monolayers in parallel with imaging of cAMP and PKA activity using FRET-based reporters in single cells. [Am J Physiol Gastrointest Liver Physiol] Abstract

Epibrassinolide Alters PI3K/MAPK Signaling Axis via Activating Foxo3a-Induced Mitochondria-Mediated Apoptosis in Colon Cancer Cells
Researchers investigated the potential apoptotic effect of epibrassinolide (EBR) on stress-related and survival signaling molecules in colon carcinoma cells. EBR decreased cell viability and colony formation in HCT 116 and HT-29 colon carcinoma cells. [Exp Cell Res] Abstract

3-O-(2′E,4′Z-Decadienoyl)-20-O-Acetylingenol Induces Apoptosis in Intestinal Epithelial Cells of Rats via Mitochondrial Pathway
The authors elucidated the cytotoxic mechanism of 3-O-(2′E,4′Z-decadienoyl)-20-O-acetylingenol in intestinal epithelial cells of rats to guide safer application of this herb in the clinic. [J Ethnopharmacol] Abstract

Uptake of Gold Nanoparticles by Intestinal Epithelial Cells: Impact of Particle Size on Their Absorption, Accumulation, and Toxicity
Scientists determined the influence of particle size and concentration of gold nanoparticles (AuNPs) on their absorption, accumulation, and cytotoxicity in model intestinal epithelial cells. [J Agric Food Chem]
Abstract | Graphical Abstract

Bisphenol A at the Reference Level Counteracts Doxorubicin Transcriptional Effects on Cancer Related Genes in HT29 Cells
Researchers aimed to assess potential interactions between bisphenol A (BPA) and doxorubicin (DOX) on HT29 colon cancer cells. HT29 cell response was evaluated after exposure to BPA, DOX, or co-exposure to both chemicals. [Toxicol In Vitro] Abstract

Down-Regulation of MicroRNA-381 Promotes Cell Proliferation and Invasion in Colon Cancer through Up-Regulation of LRH-1
The authors found that microRNA (miR)-381 was significantly down-regulated in human colon cancer tissues, compared with adjacent normal tissues. Introduction of miR-381 antisense oligos into SW480 and HCT116 cells promoted cell proliferation and invasion. [Biomed Pharmacother] Abstract


Efficient Genetic Engineering of Human Intestinal Organoids Using Electroporation
Scientists describe a detailed protocol for the generation of intestinal organoids by culture with essential growth factors in a basement membrane matrix. [Nat Protoc] Full Article

Bunched and Madm Function Downstream of Tuberous Sclerosis Complex to Regulate the Growth of Intestinal Stem Cells in Drosophila
Investigators showed that the leucine zipper protein Bunched and the adaptor protein Madm are novel regulators of intestinal stem cells. [Stem Cell Rev] Full Article

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Colorectal Cancer Models for Novel Drug Discovery
The authors review established models of in vitro cell culture and describe advances in organoid culture for studying normal and malignant intestine. They also discuss key features of classic xenograft models and describe other approaches for in vivo colorectal cancer research, including patient-derived xenograft, carcinogen-induced, orthotopic transplantation and transgenic mouse models. [Expert Opin Drug Discov] Abstract

Visit our reviews page to see a complete list of reviews in the intestinal cell research field.
Results Of Confirmatory Clinical Trial Presented Support IBgard® As A New Approach In Managing Irritable Bowel Syndrome (IBS)
The clinical trial entitled IBSRRET™ (Irritable Bowel Safety and Response Rate Evaluation Trial) demonstrated a significant decrease in intensity of symptoms, including abdominal pain and discomfort, bloating, constipation, diarrhea, sense of incomplete evacuation and urgency. [Press release from IBgard® discussing research presented at the 2015 James W. Freston Conference: A Renaissance in the Understanding and Managing of Irritable Bowel Syndrome, Chicago] Press Release

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Successful BNC101 IND Submission Paves Way for Development of Promising Cancer Stem Cell Drug Candidate
Bionomics Limited announced that its BNC101 IND submission has passed review by the US Food and Drug Administration (FDA). Bionomics plans to initiate a Phase I clinical trial in patients with metastatic colon cancer and in patients with metastatic pancreatic cancer prior to 31 December 2015. [Bionomics Limited] Press Release

Regen BioPharma, Inc. BORIS Gene Silencing Technology Successfully Utilized by Independent Researchers to Kill Breast Cancer and Colon Cancer Stem Cells
Regen BioPharma Inc. reported the successful implementation of its gene silencing technology, covered by patent #8,263,571, in killing cancer stem cells. Researchers had reported that blocking the BORIS gene results in a profound reduction of breast cancer and colon cancer stem cells. [Regen BioPharma Inc. (PR Newswire Association LLC)] Press Release

Glenmark’s Novel Monoclonal Antibody GBR 830 to Enter Phase II Clinical Studies in Atopic Dermatitis and Celiac Disease in US and Europe
Glenmark Pharmaceuticals S.A. announced that GBR 830, a novel monoclonal antibody has completed clinical Phase I dosing. GBR 830 is an antagonist of OX40, a costimulatory receptor expressed on T cells mediating T cell activation and survival. [Glenmark Pharmaceuticals S.A. (PR Newswire Association LLC)] Press Release

UC San Diego Cancer Researchers Receive NCI Outstanding Investigator Award
Four University of California, San Diego School of Medicine researchers have been selected to receive the newly established National Cancer Institute (NCI) Outstanding Investigator Award. The multi-million dollar awards fund new projects that have an unusual potential in cancer research over seven years. [UC San Diego] Press Release
National Institutes of Health (United States)

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)
NEW Keystone Symposia: Stem Cells and Regeneration in the Digestive Organs
March 13-17, 2016
Keystone, United States

Visit our events page to see a complete list of events in the intestinal cell research community.
NEW Postdoctoral Research Fellow – Colorectal Cancer (Fred Hutchinson Cancer Research Center)

Research Associate – Intestinal Cell Biology (STEMCELL Technologies Inc.)

Scientist – Pluripotent Stem Cell Biology Endoderm Lineages (STEMCELL Technologies Inc.)

Postdoctoral Position – Drug Discovery and Action (University of Illinois)

Postdoctoral or PhD position – Intestinal Lipidomics (Max Planck Institute of Molecular Cell Biology and Genetics)

Senior Research Technician (Qu Biologics Inc.)

Genetic Counselor – Cancer (Fred Hutchinson Cancer Research Center)

Harry Eagle Scholar Awards for Postdoctoral Candidates (Albert Einstein College of Medicine of Yeshiva University)

Postdoctoral Research Fellow – Ex Vivo Tumor Organoid Models (Fred Hutchinson Cancer Research Center)

Postdoctoral Positions – Cancer Research (Rutgers University)

Postdoctoral Researcher – Immunology and Microbiology (New York University School of Medicine)

Postdoctoral Position – Intestinal Immunology (Shanghai Institute of Immunology)

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